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Pathology Core
The Pathology Core will be used by all of the research projects of the Lovelace-CRCE. This core will coordinate and provide services for necropsy, specimen collection, clinical hematology and serum chemistry, tissue fixation and processing, preparation of histology specimens, and histopathologic analyses. For Project 5, the core will collect and process tissues and body fluids under standard operating procedures (SOPs) using Good Laboratory Practices (GLPs) as outlined by the U.S. Food and Drug Administration (FDA).
Specimens obtained by the core in these projects may be analyzed by clinical pathology and histopathology as well as by methods outlined in Project 1 for identification of potential targets of therapeutic intervention. Specific methods for study will include but are not limited to:
Routine necropsy procedures for rodents
Routine hematology, serum chemistry, and urinalysis for toxicity assessment
Bronchoalveolar lavage (BAL) for analysis of cells and fluid
Constant pressure inflation-fixation of lungs for stereology/morphometry
Standard and special staining of paraffin embedded tissues
Quantitative morphometry for pulmonary emphysema and fibrosis
Additional procedures may include cryomicrotomy, immunohistochemistry for any antigen in frozen or paraffin-embedded tissue sections, and in situ hybridization for mRNA detection.
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